Ultimate Guide to Peptide Reconstitution
Reconstitution quality controls both dosing accuracy and downstream stability. This guide outlines a clean, repeatable workflow.
Solvent Selection
Bacteriostatic Water (Preferred)
- Supports multi-dose handling workflows — 0.9% benzyl alcohol prevents microbial growth
- Typically preferred for practical stability in multi-access vials
Source: DailyMed — Bacteriostatic Water for Injection (0.9% benzyl alcohol). CDC Injection Safety guidance.
Saline (0.9% NaCl)
- Can be used in select workflows (e.g., benzyl alcohol allergy)
- Often associated with faster effective decay than BAC handling — estimated 1.5× decay rate in PeptideClock model [Model Estimate — PeptideClock; no direct peptide-specific assay exists]
Sterile Water
- No preservative component — single-dose use only
- Usually shortest practical handling window once opened
Source: DailyMed — Sterile Water for Injection (no preservative, single-dose).
Evidence Basis
Concentration Cheat Sheet
| Vial Amount | Volume Added | Concentration |
|---|---|---|
| 5mg | 2ml | 2.5mg/ml (2500mcg/ml) |
| 10mg | 2ml | 5.0mg/ml (5000mcg/ml) |
| 5mg | 5ml | 1.0mg/ml (1000mcg/ml) |
| 15mg | 3ml | 5.0mg/ml (5000mcg/ml) |
Common Mistakes
- Shaking instead of gentle swirling
- Injecting solvent directly onto powder puck
- Poor stopper sanitation and handling hygiene
- No concentration label after reconstitution